Research Use Only · Dosing data compiled from published literature · Not for human or veterinary use

PNC-27 Dosage & Reconstitution

p53-derived HDM-2 binding peptide. Work out exactly what to draw for your own mix, see the doses used in research, and follow a week-by-week plan. Evidence tier: preclinical.

1Set Up Your Mix

3.333 mg/mL · 3333 mcg/mL

Your Answer — PNC-27

10 mg in 3 mL
3Draw To This Line
0.03That's This Many mL
100Doses In The Vial
0102030405060708090100 U-100 syringe · 1 mL · 100 units draw to 3
10 mg ÷ 3 mL = 3.333 mg/mL  →  100 mcg ÷ 3.333 mg/mL = 0.03 mL  →  × 100 = 3 units
Looks good. Draw to 3 units on a 1 mL syringe. That is an easy amount to measure, and this vial gives you 100 doses.
When to dose
Time of dayNo particular time of day has been established.
FoodNo fasting requirement has been established.

The studies behind this compound did not test timing or food state as variables, so we are not going to invent a rule. Pick a time you can keep to consistently.

2Week-By-Week Plan — PNC-27

Gradual daily titration
WhenDoseHow OftenHow Many mLDraw ToNumber Of DosesPeptide Used
Weeks 1-4
start here
100 mcg daily 0.03 mL 3 u 28 2.8 mg
Weeks 5-8 250 mcg daily 0.075 mL 7.5 u 28 7 mg
Weeks 9 onward most stop here
per 4 weeks from here on
500 mcg daily 0.15 mL 15 u 28 14 mg
Time to get there
8 weeks to the 500 mcg dose
Peptide used reaching that
9.8 mg
Vials you'll need
1 × 10 mg
Ongoing, once you settle
14 mg / 4 wk · 1.4 vials
Where this plan came from
A published protocol
Where this came from: The peptidedosages.com protocol - 100 mcg daily, working up to 500 mcg. No authoritative human dosing exists for PNC-27, and the FDA has warned that its safety has not been established. Treat every figure here as an educational protocol rather than an established dose.

3Common Doses — PNC-27

worked out for your mix
LevelDoseHow OftenHow Many mLDraw ToDoses In Vial
Low (start) 100 mcg once daily 0.03 mL 3 u 100 doses
Commonly reported 250 mcg once daily 0.075 mL 7.5 u 40 doses
Upper reported 500 mcg once daily 0.15 mL 15 u 20 doses
Please read this. These numbers come from published research. They are here as reference, not as instructions. A lot of them come from lab studies rather than studies in people, and some of these peptides have never been given to a person at all.

About PNC-27

Immune & Anti-microbial
Also known as
p53-derived HDM-2 binding peptide
Class
Immune & Anti-microbial
Route
Subcutaneous
Half-life
Short
Evidence tier
Preclinical

A peptide combining a p53 fragment with a penetratin sequence, studied in cancer cell models for selective membrane disruption. Dosed daily in micrograms, not milligrams. No authoritative human dosing exists and the FDA has warned that its safety has not been established.

Buy PNC-27

Common Questions — PNC-27

How much bacteriostatic water do I add to a 10 mg vial of PNC-27?

Adding 3 mL of bacteriostatic water to a 10 mg vial gives 3.33 mg/mL. That is the mix every number on this page is worked out from — change the water figure in the calculator and all the draw amounts update.

What dose of PNC-27 is used in research?

Published and reported figures for PNC-27 run from 10 mg, typically variable. This site lists each figure with the source it came from rather than giving a single recommended number.

How many units on the syringe is a 100 mcg dose of PNC-27?

At 3.33 mg/mL, a 100 mcg dose is 3 units on a 1 mL U-100 insulin syringe. One vial at that mix gives about 100 doses.

How strong is the evidence behind these PNC-27 doses?

PNC-27 is graded preclinical. Laboratory and animal data only. No human dosing trials have been published. We grade every compound on this site so you can weigh the numbers accordingly — the full method is on our editorial policy page.

Where These Numbers Come From

Preclinical — Preclinical and in-vitro data only — no human dosing trials
PNC-27 induces cancer cell necrosis via HDM-2 membrane interaction Michl J, Sarafraz-Yazdi E, Pincus MR, et al. Journal of Molecular Medicine / PLOS ONE, 2006–2019
The originating and follow-up in vitro and preclinical work.
Find on PubMed ↗

Newest Studies —

Not reviewed by us. This list is pulled straight from Europe PMC as papers are published. Nobody has checked whether these are any good, or whether they change anything above. The dose numbers on this page only ever change after a person reviews the evidence.

Other immune & anti-microbial peptides

Compounds in the same class, or browse the full catalogue.

LL-37

The sole human cathelicidin-derived antimicrobial peptide. Well characterised in immunology literature for direct antimicrobial an…

Thymosin Alpha-1

A thymic peptide approved in a number of countries as an immunomodulator, principally as adjunct therapy in chronic hepatitis B an…

All 72 peptides

Every compound compared in one table: dose range, how often, half-life and evidence tier.

Need this on one page to print, screenshot or send?

Make a dose card →