Research Use Only · Dosing data compiled from published literature · Not for human or veterinary use

NAD+ Dosage & Reconstitution

Nicotinamide adenine dinucleotide. Work out exactly what to draw for your own mix, see the doses used in research, and follow a week-by-week plan. Evidence tier: limited.

1Set Up Your Mix

100 mg/mL · 100000 mcg/mL

Your Answer — NAD+

500 mg in 5 mL
50Draw To This Line
0.5That's This Many mL
10Doses In The Vial
0102030405060708090100 U-100 syringe · 1 mL · 100 units draw to 50
500 mg ÷ 5 mL = 100 mg/mL  →  50 mg ÷ 100 mg/mL = 0.5 mL  →  × 100 = 50 units
Looks good. Draw to 50 units on a 1 mL syringe. That is an easy amount to measure, and this vial gives you 10 doses.
When to dose
Time of dayNo particular time of day has been established.
FoodNo fasting requirement has been established.

The studies behind this compound did not test timing or food state as variables, so we are not going to invent a rule. Pick a time you can keep to consistently.

2Week-By-Week Plan — NAD+

Start low, work up
WhenDoseHow OftenHow Many mLDraw ToNumber Of DosesPeptide Used
Weeks 1–2 50 mg daily 0.5 mL 50 u 14 700 mg
Week 3 onward
per 4 weeks from here on
100 mg daily 1 mL 100 u 28 2800 mg
Time until the top dose
2 weeks
Peptide used getting there
700 mg
Vials you'll need
2 × 500 mg
Once you're at the top dose
2800 mg / 4 wk · 5.6 vials
Where this plan came from
Built from this peptide's dose range
Where this came from: Built from the doses listed for this peptide below — start at the lowest and step up, using the studies cited on this page. Nobody publishes a week-by-week plan for this peptide, so we built one: the doses are the researched ones shown below, and we simply put them in order from lowest to highest. Treat it as a starting point, not an official schedule.

3Common Doses — NAD+

worked out for your mix
LevelDoseHow OftenHow Many mLDraw ToDoses In Vial
Low reported (SC) 50 mg 1× daily 0.5 mL 50 u 10 doses
Standard (SC) 100 mg 1× daily 1 mL 100 u 5 doses
Upper reported range250–1000 mg over several hoursperiodic Set by body weight — can't be turned into a single amount to draw
Please read this. These numbers come from published research. They are here as reference, not as instructions. A lot of them come from lab studies rather than studies in people, and some of these peptides have never been given to a person at all.

About NAD+

Mitochondrial
Also known as
Nicotinamide adenine dinucleotide
Class
Mitochondrial
Route
Subcutaneous
Half-life
Rapid clearance
Evidence tier
Limited

A coenzyme rather than a peptide. Direct NAD+ administration is bioavailability-limited and often poorly tolerated (stinging, flushing, nausea). Most rigorous human research uses precursors — NR or NMN — rather than NAD+ itself.

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Common Questions — NAD+

How much bacteriostatic water do I add to a 500 mg vial of NAD+?

Adding 5 mL of bacteriostatic water to a 500 mg vial gives 100 mg/mL. That is the mix every number on this page is worked out from — change the water figure in the calculator and all the draw amounts update.

What dose of NAD+ is used in research?

Published and reported figures for NAD+ run from 50 mg to 100 mg, typically 1× daily. This site lists each figure with the source it came from rather than giving a single recommended number.

How many units on the syringe is a 50 mg dose of NAD+?

At 100 mg/mL, a 50 mg dose is 50 units on a 1 mL U-100 insulin syringe. One vial at that mix gives about 10 doses.

How strong is the evidence behind these NAD+ doses?

NAD+ is graded limited. The figures are sparse — a single programme or community-reported numbers. We grade every compound on this site so you can weigh the numbers accordingly — the full method is on our editorial policy page.

Where These Numbers Come From

Limited — Sparse, single-programme, or community-reported figures
NAD+ metabolism and its roles in cellular processes during ageing Covarrubias AJ, et al. Nature Reviews Molecular Cell Biology, 2021
The definitive current review of NAD+ biology and where the evidence actually stands.
Find on PubMed ↗
Nicotinamide riboside supplementation in humans — dose escalation Trammell SAJ, et al. Nature Communications, 2016
Human PK for the precursor route, which is far better characterised than direct NAD+.
Find on PubMed ↗
Chronic nicotinamide riboside supplementation is well tolerated Martens CR, et al. Nature Communications, 2018
Human safety and tolerability at defined doses.
Find on PubMed ↗

Newest Studies —

Not reviewed by us. This list is pulled straight from Europe PMC as papers are published. Nobody has checked whether these are any good, or whether they change anything above. The dose numbers on this page only ever change after a person reviews the evidence.

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