Research Use Only · Dosing data compiled from published literature · Not for human or veterinary use

Glutathione Dosage & Reconstitution

GSH, reduced glutathione. Work out exactly what to draw for your own mix, see the doses used in research, and follow a week-by-week plan. Evidence tier: clinical.

1Set Up Your Mix

100 mg/mL · 100000 mcg/mL

Your Answer — Glutathione

500 mg in 5 mL
50Draw To This Line
0.5That's This Many mL
10Doses In The Vial
0102030405060708090100 U-100 syringe · 1 mL · 100 units draw to 50
500 mg ÷ 5 mL = 100 mg/mL  →  50 mg ÷ 100 mg/mL = 0.5 mL  →  × 100 = 50 units
Looks good. Draw to 50 units on a 1 mL syringe. That is an easy amount to measure, and this vial gives you 10 doses.
When to dose
Time of dayNo particular time of day has been established.
FoodNo fasting requirement has been established.

The studies behind this compound did not test timing or food state as variables, so we are not going to invent a rule. Pick a time you can keep to consistently.

2Week-By-Week Plan — Glutathione

Step up to 200 mg and stay there
WhenDoseHow OftenHow Many mLDraw ToNumber Of DosesPeptide Used
Weeks 1–2
start here
50 mg twice weekly 0.5 mL 50 u 4 200 mg
Weeks 3–4 100 mg twice weekly 1 mL 100 u 4 400 mg
Week 5 onward most stop here
stay at this dose
200 mg twice weekly 2 mL 200 u ⚠ 4 800 mg
Time to get there
4 weeks to the maintenance dose
Peptide used reaching that
600 mg
Vials you'll need
2 × 500 mg
Ongoing, once you settle
800 mg / 4 wk · 1.6 vials
Where this plan came from
A published protocol
Where this came from: OptimalDosages protocol. Two weeks at each step, then hold at 200 mg. There is no reason to keep climbing past that on this protocol.

3Common Doses — Glutathione

worked out for your mix
LevelDoseHow OftenHow Many mLDraw ToDoses In Vial
Weeks 1–2 50 mg 2–3× weekly 0.5 mL 50 u 10 doses
Weeks 3–4 100 mg 2–3× weekly 1 mL 100 u 5 doses
Week 5 onward 200 mg 2–3× weekly 2 mL 200 u ⚠ 2 doses
Please read this. These numbers come from published research. They are here as reference, not as instructions. A lot of them come from lab studies rather than studies in people, and some of these peptides have never been given to a person at all.

About Glutathione

Mitochondrial
Also known as
GSH, reduced glutathione
Class
Mitochondrial
Route
Subcutaneous
Half-life
Short (minutes)
Evidence tier
Clinical

The principal endogenous intracellular antioxidant tripeptide. Widely administered in clinical settings; the evidence for parenteral supplementation improving outcomes in healthy subjects is thin.

Buy Glutathione

Common Questions — Glutathione

How much bacteriostatic water do I add to a 500 mg vial of Glutathione?

Adding 5 mL of bacteriostatic water to a 500 mg vial gives 100 mg/mL. That is the mix every number on this page is worked out from — change the water figure in the calculator and all the draw amounts update.

What dose of Glutathione is used in research?

Published and reported figures for Glutathione on this protocol run from 50 mg to 200 mg, stepping up over four weeks and holding at 200 mg. This site lists each figure with the source it came from rather than giving a single recommended number.

How many units on the syringe is a 50 mg dose of Glutathione?

At 100 mg/mL, a 50 mg dose is 50 units on a 1 mL U-100 insulin syringe. One vial at that mix gives about 10 doses.

How strong is the evidence behind these Glutathione doses?

Glutathione is graded clinical. Human trial or approved-label dosing has been published for this compound. We grade every compound on this site so you can weigh the numbers accordingly — the full method is on our editorial policy page.

Where These Numbers Come From

Clinical — Human trial or approved-label dosing published
Glutathione — metabolism and physiological functions Forman HJ, Zhang H, Rinna A. Molecular Aspects of Medicine, 2009
Standard biochemical reference.
Find on PubMed ↗
Randomised controlled trial of oral glutathione supplementation Richie JP, et al. European Journal of Nutrition, 2015
One of the few controlled human supplementation trials.
Find on PubMed ↗

Newest Studies —

Not reviewed by us. This list is pulled straight from Europe PMC as papers are published. Nobody has checked whether these are any good, or whether they change anything above. The dose numbers on this page only ever change after a person reviews the evidence.

Other mitochondrial peptides

Compounds in the same class, or browse the full catalogue.

MOTS-c

A mitochondrial-derived peptide acting via AMPK on insulin sensitivity and metabolic homeostasis. Genuinely interesting mechanism …

NAD+

A coenzyme rather than a peptide. Direct NAD+ administration is bioavailability-limited and often poorly tolerated (stinging, flus…

All 72 peptides

Every compound compared in one table: dose range, how often, half-life and evidence tier.

Need this on one page to print, screenshot or send?

Make a dose card →