Thymosin Beta-4 fragment (Ac-SDKP region). Work out exactly what to draw for your own mix, see the doses used in research, and follow a week-by-week plan. Evidence tier: preclinical.
The studies behind this compound did not test timing or food state as variables, so we are not going to invent a rule. Pick a time you can keep to consistently.
| When | Dose | How Often | How Many mL | Draw To | Number Of Doses | Peptide Used |
|---|---|---|---|---|---|---|
| Weeks 1–2 start here |
500 mcg | daily | 0.15 mL | 15 u | 14 | 7 mg |
| Weeks 3–4 | 600 mcg | daily | 0.18 mL | 18 u | 14 | 8.4 mg |
| Weeks 5–8 | 750 mcg | daily | 0.225 mL | 22.5 u | 28 | 21 mg |
| Weeks 9–12 most stop here most stay here |
1000 mcg | daily | 0.3 mL | 30 u | 28 | 28 mg |
| Level | Dose | How Often | How Many mL | Draw To | Doses In Vial |
|---|---|---|---|---|---|
| Weeks 1–2 | 500 mcg | once daily | 0.15 mL | 15 u | 20 doses |
| Weeks 3–4 | 600 mcg | once daily | 0.18 mL | 18 u | 16 doses |
| Weeks 5–8 | 750 mcg | once daily | 0.225 mL | 22.5 u | 13 doses |
| Weeks 9–12 | 1000 mcg | once daily | 0.3 mL | 30 u | 10 doses |
A synthetic fragment of thymosin beta-4, the actin-sequestering protein. Full-length Tβ4 reached human trials for dry eye and cardiac indications; the truncated research fragment sold as TB-500 has not. Dosing conventions come from the research community, loosely anchored to preclinical Tβ4 work.
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Adding 3 mL of bacteriostatic water to a 10 mg vial gives 3.33 mg/mL. That is the mix every number on this page is worked out from — change the water figure in the calculator and all the draw amounts update.
Published and reported figures for TB-500 on this protocol run from 500 mcg to 1000 mcg daily, stepping up over twelve weeks. This site lists each figure with the source it came from rather than giving a single recommended number.
At 3.33 mg/mL, a 500 mcg dose is 15 units on a 1 mL U-100 insulin syringe. One vial at that mix gives about 20 doses.
TB-500 is graded preclinical. Laboratory and animal data only. No human dosing trials have been published. We grade every compound on this site so you can weigh the numbers accordingly — the full method is on our editorial policy page.
Yes — Loading and maintenance phases. The week-by-week table on this page converts each step into the exact amount to draw for your mix.
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