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Research Use Only · Dosing data compiled from published literature · Not for human or veterinary use
OptimalDosages

TB-500 Dosage & Reconstitution

Thymosin Beta-4 fragment (Ac-SDKP region). Work out exactly what to draw for your own mix, see the doses used in research, and follow a week-by-week plan. Evidence tier: preclinical.

1Set Up Your Mix

10 mg/mL · 10000 mcg/mL
All answers assume a standard 1 mL U-100 syringe, where 1 unit = 0.01 mL. That is the usual insulin syringe and the one every number here is based on.

Your Answer — TB-500Your Answer

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50Draw To This Line
0.5That's This Many mL
1Doses In The Vial
0102030405060708090100 U-100 syringe · 1 mL · 100 units draw to 50
5 mg ÷ 0.5 mL = 10 mg/mL  →  5 mg ÷ 10 mg/mL = 0.5 mL  →  × 100 = 50 units—
Looks good. Draw to 50 units on a 1 mL syringe. That is an easy amount to measure, and this vial gives you 1 doses.

2Week-By-Week Plan — TB-500

Loading and maintenance phases
WhenDoseHow OftenHow Many mLDraw ToNumber Of DosesPeptide Used
Loading phase 2 mg 2× weekly, 4–6 wk 0.2 mL 20 u 2 doses
Upper loading 5 mg weekly total, split 0.5 mL 50 u 1 doses
Maintenance 2 mg every 1–2 weeks 0.2 mL 20 u 2 doses
Tβ4 trial dose42 mg (cardiac trial)single/serial Set by body weight — can't be turned into a single amount to draw
Weeks until the top dose
6 weeks
Peptide used getting there
24 mg
Vials you'll need
5 × 5 mg
Once you're at the top dose
4 mg / 4 wk · 0.8 vials
Where this plan came from
A commonly used protocol
Where this came from: Convention within the research community — not derived from a registered trial. A loading phase followed by reduced-frequency maintenance, as the compound is commonly protocolled.

3Common Doses — TB-500

worked out for your mix
LevelDoseHow OftenHow Many mLDraw ToDoses In Vial
Loading — weeks 1–4 2 mg twice weekly 0.2 mL 20 u 8 16 mg
Loading — weeks 5–6 2 mg twice weekly 0.2 mL 20 u 4 8 mg
Maintenance — week 7 onward
per 4 weeks from here on		 2 mg every 2 weeks 0.2 mL 20 u 2 4 mg
Please read this. These numbers come from published research. They are here as reference, not as instructions. A lot of them come from lab studies rather than studies in people, and some of these peptides have never been given to a person at all.

About TB-500About This Peptide

Tissue Repair & Recovery
Also known as
Thymosin Beta-4 fragment (Ac-SDKP region)
Class
Tissue Repair & Recovery
Route
Subcutaneous
Half-life
~2–3 h (parent Tβ4)
Evidence tier
Preclinical

A synthetic fragment of thymosin beta-4, the actin-sequestering protein. Full-length Tβ4 reached human trials for dry eye and cardiac indications; the truncated research fragment sold as TB-500 has not. Dosing conventions come from the research community, loosely anchored to preclinical Tβ4 work.

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Where These Numbers Come From

Preclinical — Preclinical and in-vitro data only — no human dosing trials
Thymosin β4: actin-sequestering protein moonlights to repair injured tissues Goldstein AL, Hannappel E, Kleinman HK. Trends in Molecular Medicine, 2005
The foundational review of the repair mechanism; everything downstream traces here.
Find on PubMed ↗
Thymosin beta 4 accelerates wound healing Malinda KM, et al. Journal of Investigative Dermatology, 1999
Early controlled wound-healing demonstration in preclinical models.
Find on PubMed ↗
Thymosin beta-4 in acute myocardial infarction — clinical evaluation RegeneRx clinical programme reports, 2010s
The closest thing to human dosing data for the parent molecule; note it is IV full-length Tβ4, not TB-500.
Find on PubMed ↗

Newest Studies — —

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Not reviewed by us. This list is pulled straight from Europe PMC as papers are published. Nobody has checked whether these are any good, or whether they change anything above. The dose numbers on this page only ever change after a person reviews the evidence.

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