Research Use Only · Dosing data compiled from published literature · Not for human or veterinary use

TB-500 Dosage & Reconstitution

Thymosin Beta-4 fragment (Ac-SDKP region). Work out exactly what to draw for your own mix, see the doses used in research, and follow a week-by-week plan. Evidence tier: preclinical.

1Set Up Your Mix

3.333 mg/mL · 3333 mcg/mL

Your Answer — TB-500

10 mg in 3 mL
15Draw To This Line
0.15That's This Many mL
20Doses In The Vial
0102030405060708090100 U-100 syringe · 1 mL · 100 units draw to 15
10 mg ÷ 3 mL = 3.333 mg/mL  →  500 mcg ÷ 3.333 mg/mL = 0.15 mL  →  × 100 = 15 units
Looks good. Draw to 15 units on a 1 mL syringe. That is an easy amount to measure, and this vial gives you 20 doses.
When to dose
Time of dayNo particular time of day has been established.
FoodNo fasting requirement has been established.

The studies behind this compound did not test timing or food state as variables, so we are not going to invent a rule. Pick a time you can keep to consistently.

2Week-By-Week Plan — TB-500

Step up from 500 mcg over 12 weeks
WhenDoseHow OftenHow Many mLDraw ToNumber Of DosesPeptide Used
Weeks 1–2
start here
500 mcg daily 0.15 mL 15 u 14 7 mg
Weeks 3–4 600 mcg daily 0.18 mL 18 u 14 8.4 mg
Weeks 5–8 750 mcg daily 0.225 mL 22.5 u 28 21 mg
Weeks 9–12 most stop here
most stay here
1000 mcg daily 0.3 mL 30 u 28 28 mg
Time to get there
12 weeks
Peptide used reaching that
36.4 mg
Vials you'll need
4 × 10 mg
Ongoing, once you settle
28 mg / 4 wk · 2.8 vials
Where this plan came from
A published protocol
Where this came from: peptidedosage.org. Once daily subcutaneously. Written for a 10 mg vial in 3 mL, the largest practical dilution, which keeps every per-injection figure in a comfortable range to measure. The weekly total averages about 5 mg.

3Common Doses — TB-500

worked out for your mix
LevelDoseHow OftenHow Many mLDraw ToDoses In Vial
Weeks 1–2 500 mcg once daily 0.15 mL 15 u 20 doses
Weeks 3–4 600 mcg once daily 0.18 mL 18 u 16 doses
Weeks 5–8 750 mcg once daily 0.225 mL 22.5 u 13 doses
Weeks 9–12 1000 mcg once daily 0.3 mL 30 u 10 doses
Please read this. These numbers come from published research. They are here as reference, not as instructions. A lot of them come from lab studies rather than studies in people, and some of these peptides have never been given to a person at all.

About TB-500

Tissue Repair & Recovery
Also known as
Thymosin Beta-4 fragment (Ac-SDKP region)
Class
Tissue Repair & Recovery
Route
Subcutaneous
Half-life
~2–3 h (parent Tβ4)
Evidence tier
Preclinical

A synthetic fragment of thymosin beta-4, the actin-sequestering protein. Full-length Tβ4 reached human trials for dry eye and cardiac indications; the truncated research fragment sold as TB-500 has not. Dosing conventions come from the research community, loosely anchored to preclinical Tβ4 work.

Buy TB-500

Common Questions — TB-500

How much bacteriostatic water do I add to a 10 mg vial of TB-500?

Adding 3 mL of bacteriostatic water to a 10 mg vial gives 3.33 mg/mL. That is the mix every number on this page is worked out from — change the water figure in the calculator and all the draw amounts update.

What dose of TB-500 is used in research?

Published and reported figures for TB-500 on this protocol run from 500 mcg to 1000 mcg daily, stepping up over twelve weeks. This site lists each figure with the source it came from rather than giving a single recommended number.

How many units on the syringe is a 500 mcg dose of TB-500?

At 3.33 mg/mL, a 500 mcg dose is 15 units on a 1 mL U-100 insulin syringe. One vial at that mix gives about 20 doses.

How strong is the evidence behind these TB-500 doses?

TB-500 is graded preclinical. Laboratory and animal data only. No human dosing trials have been published. We grade every compound on this site so you can weigh the numbers accordingly — the full method is on our editorial policy page.

Is there a TB-500 titration schedule?

Yes — Loading and maintenance phases. The week-by-week table on this page converts each step into the exact amount to draw for your mix.

Where These Numbers Come From

Preclinical — Preclinical and in-vitro data only — no human dosing trials
Thymosin β4: actin-sequestering protein moonlights to repair injured tissues Goldstein AL, Hannappel E, Kleinman HK. Trends in Molecular Medicine, 2005
The foundational review of the repair mechanism; everything downstream traces here.
Find on PubMed ↗
Thymosin beta 4 accelerates wound healing Malinda KM, et al. Journal of Investigative Dermatology, 1999
Early controlled wound-healing demonstration in preclinical models.
Find on PubMed ↗
Thymosin beta-4 in acute myocardial infarction — clinical evaluation RegeneRx clinical programme reports, 2010s
The closest thing to human dosing data for the parent molecule; note it is IV full-length Tβ4, not TB-500.
Find on PubMed ↗

Newest Studies —

Not reviewed by us. This list is pulled straight from Europe PMC as papers are published. Nobody has checked whether these are any good, or whether they change anything above. The dose numbers on this page only ever change after a person reviews the evidence.

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