PE-22-28 Dosage Calculator & Doses

1Set Up Your Mix

3.333 mg/mL · 3333 mcg/mL

1. Pick your peptide

2. How much peptide is in the vial? It's printed on the label — usually something like 5 mg

3. How much water are you adding? More water makes each dose easier to measure

4. How much do you want per dose?

Your Answer — PE-22-28

10 mg in 3 mL

7.5Draw To This Line

0.075That's This Many mL

40Doses In The Vial

10 mg ÷ 3 mL = 3.333 mg/mL → 250 mcg ÷ 3.333 mg/mL = 0.075 mL → × 100 = 7.5 units

Looks good. Draw to 7.5 units on a 1 mL syringe. That is an easy amount to measure, and this vial gives you 40 doses.

When to dose

Time of dayNo particular time of day has been established.

FoodNo fasting requirement has been established.

The studies behind this compound did not test timing or food state, so we are not going to invent a rule.

2Week-By-Week Plan — PE-22-28

Start low, work up

When	Dose	How Often	How Many mL	Draw To	Number Of Doses	Peptide Used
Weeks 1–2	250 mcg	daily	0.075 mL	7.5 u	14	3.5 mg
Week 3 onward per 4 weeks from here on	500 mcg	daily	0.15 mL	15 u	28	14 mg

Time until the top dose

2 weeks

Peptide used getting there

3.5 mg

Vials you'll need

1 × 10 mg

Once you're at the top dose

14 mg / 4 wk · 1.4 vials

Where this plan came from

Built from this peptide's dose range

Where this came from: Built from the doses listed for this peptide below — start at the lowest and step up, using the studies cited on this page. Nobody publishes a week-by-week plan for this peptide, so we built one: the doses are the researched ones shown below, and we simply put them in order from lowest to highest. Treat it as a starting point, not an official schedule.

3Common Doses — PE-22-28

worked out for your mix

Level	Dose	How Often	How Many mL	Draw To	Doses In Vial
Research study dose	~0.1 mg/kg weight-based	daily	Set by body weight — can't be turned into a single amount to draw
Community reported	250 mcg	1× daily	0.075 mL	7.5 u	40 doses
Community upper	500 mcg	1× daily	0.15 mL	15 u	20 doses

Please read this. These numbers come from published research. They are here as reference, not as instructions. A lot of them come from lab studies rather than studies in people, and some of these peptides have never been given to a person at all.

About PE-22-28

Cognitive & Neuro

Also known as

Spadin analogue, TREK-1 blocker

Class

Cognitive & Neuro

Route

Subcutaneous

Half-life

Short

Evidence tier

Preclinical

A shortened spadin analogue that blocks the TREK-1 potassium channel, proposed as a fast-acting antidepressant mechanism. Preclinical data only.

Buy PE-22-28

Research Equipment

SyringesU-100 insulin · 1 mL · 29–31G

Alcohol wipes70% isopropyl prep pads

Serum base — for topical mixesHyaluronic acid serum for mixing peptides into a serum

Electric cooler caseTSA-approved travel cooler — keeps mixed vials at fridge temperature

Storage caseHard carry case for vials

Paid affiliate links.

Common Questions — PE-22-28

How much bacteriostatic water do I add to a 10 mg vial of PE-22-28?

Adding 3 mL of bacteriostatic water to a 10 mg vial gives 3.33 mg/mL. That is the mix every number on this page is worked out from — change the water figure in the calculator and all the draw amounts update.

What dose of PE-22-28 is used in research?

Published and reported figures for PE-22-28 run from 250 mcg to 500 mcg, typically 1× daily. This site lists each figure with the source it came from rather than giving a single recommended number.

How many units on the syringe is a 250 mcg dose of PE-22-28?

At 3.33 mg/mL, a 250 mcg dose is 7.5 units on a 1 mL U-100 insulin syringe. One vial at that mix gives about 40 doses.

How strong is the evidence behind these PE-22-28 doses?

PE-22-28 is graded preclinical. Laboratory and animal data only. No human dosing trials have been published. We grade every compound on this site so you can weigh the numbers accordingly — the full method is on our editorial policy page.

Where These Numbers Come From

Preclinical — Preclinical and in-vitro data only — no human dosing trials

Spadin and shortened analogues as TREK-1 blockers with antidepressant properties Veyssiere J, Moha ou Maati H, et al. British Journal of Pharmacology, 2015

The originating pharmacology; source of PE-22-28 specifically.

Find on PubMed ↗

Newest Studies — —

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Not reviewed by us. This list is pulled straight from Europe PMC as papers are published. Nobody has checked whether these are any good, or whether they change anything above. The dose numbers on this page only ever change after a person reviews the evidence.

PE-22-28 Dosage & Reconstitution