1Set Up Your Mix
40 mg/mL · 40000 mcg/mLYour Answer — Mito Blend
120 mg in 3 mLThe studies behind this compound did not test timing or food state as variables, so we are not going to invent a rule. Pick a time you can keep to consistently.
2Week-By-Week Plan — Mito Blend
Build up to 1 mg MOTS-c daily| When | Dose | How Often | How Many mL | Draw To | Number Of Doses | Peptide Used |
|---|---|---|---|---|---|---|
| Weeks 1–2 | 3 mg | daily | 0.075 mL | 7.5 u | 14 | 42 mg |
| Weeks 3–4 | 6 mg | daily | 0.15 mL | 15 u | 14 | 84 mg |
| Weeks 5–6 | 9 mg | daily | 0.225 mL | 22.5 u | 14 | 126 mg |
| Week 7 onward per 4 weeks from here on |
12 mg | daily | 0.3 mL | 30 u | 28 | 336 mg |
3Common Doses — Mito Blend
worked out for your mix| Level | Dose | How Often | How Many mL | Draw To | Doses In Vial |
|---|---|---|---|---|---|
| Week 1–2 start | 3 mg | 1× daily (0.25 mg MOTS-c / 2.5 mg NAD+) | 0.075 mL | 7.5 u | 40 doses |
| Week 3–4 | 6 mg | 1× daily (0.5 mg MOTS-c / 5 mg NAD+) | 0.15 mL | 15 u | 20 doses |
| Week 5–6 | 9 mg | 1× daily (0.75 mg MOTS-c / 7.5 mg NAD+) | 0.225 mL | 22.5 u | 13 doses |
| Target | 12 mg | 1× daily (1 mg MOTS-c / 10 mg NAD+) | 0.3 mL | 30 u | 10 doses |
About Mito Blend
—A metabolic combination in a 120 mg vial, dosed daily and anchored to MOTS-c. MOTS-c is 8.3% of the contents, so a 1 mg daily MOTS-c dose means 12 mg of blend — which also delivers 10 mg of NAD+ and 1 mg of 5-Amino-1MQ. Because the ratio is fixed, anchoring to MOTS-c puts the other two low against their own figures: NAD+ is reported at 50–100 mg per dose and 5-Amino-1MQ at 2.5–5 mg. At 1 mg MOTS-c daily a vial runs about 10 days.
Buy Mito Blend
SyringesU-100 insulin · 1 mL · 29–31G
Alcohol wipes70% isopropyl prep pads
Serum base — for topical mixesHyaluronic acid serum for mixing peptides into a serum
Electric cooler caseTSA-approved travel cooler — keeps mixed vials at fridge temperature
Storage caseHard carry case for vialsPaid affiliate links.
Common Questions — Mito Blend
How much bacteriostatic water do I add to a 120 mg vial of Mito Blend?
Adding 3 mL of bacteriostatic water to a 120 mg vial gives 40 mg/mL. That is the mix every number on this page is worked out from — change the water figure in the calculator and all the draw amounts update.
What dose of Mito Blend is used in research?
Published and reported figures for Mito Blend run from 3 mg to 12 mg, typically 1× daily (0.25 mg MOTS-c / 2.5 mg NAD+). This site lists each figure with the source it came from rather than giving a single recommended number.
How many units on the syringe is a 3 mg dose of Mito Blend?
At 40 mg/mL, a 3 mg dose is 7.5 units on a 1 mL U-100 insulin syringe. One vial at that mix gives about 40 doses.
How strong is the evidence behind these Mito Blend doses?
Mito Blend is graded preclinical. Laboratory and animal data only. No human dosing trials have been published. We grade every compound on this site so you can weigh the numbers accordingly — the full method is on our editorial policy page.
Is there a Mito Blend titration schedule?
Yes — Build up to 1 mg MOTS-c daily. The week-by-week table on this page converts each step into the exact amount to draw for your mix.
Why these three are combined
120 mg vial- NAD+ 100 mg
- MOTS-c 10 mg
- 5-Amino-1MQ 10 mg
Three different points on the same pathway. NAD+ supplies the cofactor directly. MOTS-c is a mitochondrial-derived peptide studied for AMPK signalling. 5-Amino-1MQ inhibits NNMT, which is studied as a way of preserving the NAD+ salvage pathway rather than supplying NAD+ itself.
So one component adds the cofactor, one signals, and one slows its depletion. Of the blends here this has the most coherent mechanistic story — though as always, the case rests on the individual compounds rather than a trial of the combination.